Agarose

Description : Agarose is a neutral polysaccharide extracted from certain seaweeds. The molecular structure of this polysaccharid  

Description : Agarose is a neutral polysaccharide extracted from certain seaweeds. The molecular structure of this polysaccharide gives the capacity to form gels that are very strong even at low concentrations. Agarose is the most popular medium for electrophoresis

Application: Agarose is the most popular medium for electrophoresis because of the large size for rapid diffusion and low background.

Storage : store at room temperature. Keep dry.

Features:

  +Easy preparation of gel by standard boiling or microwaving

  +High gel strength make it easy to handle gel without fracturing even at lower concentration

  +Low EEO reduces disruption of DNA separation

Electroendosmosis ( EEO) is one of the most important characteristics of agarose. Anionic group in agarose gel are affixed to the matrix and can not move, but dissociable counter cations can migrate towards the cathode in the matrix, giving rise to EEO, EEO can disrupt separations of DNA because of internal convection .The lower EEO, the faster DNA will migrate, additionally, lower EEO helps to improve the resolution of DNA and RNA as their migration is determined only by their size, not by their charge.

  +Low background during fluorescent staining

  +No detectable nuclease and protease

Specification

Product Name : Agarose

CAS No.: 9012-36-6

Appearance: white powder

Solubility: clear colorless solution at 1G plus 100ml water

Water: ≤ 7%

Gel strength: >1200 g/cm 2 (1% Gel)

EEO: <0.13

Sulfate: ≤ 0.20%

Gelling point: 3 6±1.5 1.5% Gel

Melting point: 88 ± 1.5 1.5% Gel

DNase: NONE

RNase: NONE

Protease: NONE

Endonuclease/ligase inhibitory factor: NONE

Packaging: 5kg,10kg, 15kg/carton or as per clients' requirements

Agarose